Proximity Ligation Assay:Article Title: An essential role for Argonaute 2 in EGFR-KRAS signaling in pancreatic cancer development
Article Snippet: .. Supplementary Table 1: Antibodies used in the study # Antibody Vendor Catalog Number Application Specificity 1 Anti-Ras clone 10 (RAS10) Millipore 05-516 IP (4ug), IB (1:1000), PLA (1:250) human, mouse 2 K-Ras-2B Antibody (C-19) Santa Cruz sc-521 IP (4ug) human 3 K-Ras monoclonal Ab Santa Cruz sc-30 IB (1:200) human, mouse 4 RAS (G12D Mutant Specific) DH87 Cell Signaling 14429S IB (1:1000) human 5 KRAS (3B10-2F2) Sigma- aldrich WH0003845M1 IB (1:1000) Human 6 HRAS- specific Antibody Proteintech 18295-1-AP IB (1:1000) human, mouse, rat 7 AGO2, 11A9 Sigma SAB4200085 IP (10ug) ,IB (1:1000) human 8 AGO2 EIF2C2 Sino Biologicals 50683-R036 IB (1:2000), IHC (1:100), PLA (1:200) human 9 Anti- EGFR ( phospho Y1092) Abcam ab40815 ( EP774Y) IHC (1:100) human, mouse 10 Phospho-EGF Receptor (Y1068) Cell Signaling 2234S IB (1:1000) human, mouse 11 Phospho-p44/42 MAPK (Erk 1/2) Cell Signaling 4376 IB (1:1000) human, mouse 12 ERK1(K-23) Santa Cruz sc-94 IB (1:500) human, mouse, rat 13 Total p44/42 MAPK (Erk 1/2) Cell Signaling 9102 IB (1:1000) human, mouse 14 EGFR Antibody Abcam ab52894 ( EP38Y) IB (1:1000),IHC (1:50) human, mouse, rat 15 EGFR(1005) Santa Cruz sc-03 IB (1:500) human, mouse, rat 16 Anti-FLAG antibody Sigma F7425 IP (4ug), IB (1:1000) human, mouse 17 Phospho-Akt S473 Cell Signaling 4060S IB (1:1000) human,mouse 18 AKT (pan) - C67E7 Cell Signaling 4691 IB (1:1000) human,mouse 19 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:1000) mouse 20 E-cadherin (36) Ventana Roche 790-4497 IF ( 1:100) mouse 21 Nk1.1 (PK136) Invitrogen MA1-70100 IHC (1:100) mouse 22 anti-CD4 (SP35) Ventana 790-4423 IHC (1:100) mouse 23 anti-CD68 (KP-1) Ventana 790-2931 IHC (1:100) mouse 24 anti-CD8 (SP57) Ventana 790-4460 IHC (1:100) mouse 25 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:500) mouse 26 GAPDH- HRP Cell Signaling 3683 IB (1:5000) human, mouse 27 Normal mouse IgG Santa Cruz sc-2025 Isotype Control IP mouse 28 Normal Rat IgG Abcam ab18450 Isotype Control IP rat 29 Normal Rabbit IgG Millipore 12-370 Isotype Control IP rabbit IP: Immunoprecipitation; IB: Immunoblot; IHC: Immunohistochemistry; PLA: Proximity Ligation Assay Validation of the antibodies used in the study: References and links cited below show specificity and use of the respective antibody for various applications. ..
Mutagenesis:Article Title: An essential role for Argonaute 2 in EGFR-KRAS signaling in pancreatic cancer development
Article Snippet: .. Supplementary Table 1: Antibodies used in the study # Antibody Vendor Catalog Number Application Specificity 1 Anti-Ras clone 10 (RAS10) Millipore 05-516 IP (4ug), IB (1:1000), PLA (1:250) human, mouse 2 K-Ras-2B Antibody (C-19) Santa Cruz sc-521 IP (4ug) human 3 K-Ras monoclonal Ab Santa Cruz sc-30 IB (1:200) human, mouse 4 RAS (G12D Mutant Specific) DH87 Cell Signaling 14429S IB (1:1000) human 5 KRAS (3B10-2F2) Sigma- aldrich WH0003845M1 IB (1:1000) Human 6 HRAS- specific Antibody Proteintech 18295-1-AP IB (1:1000) human, mouse, rat 7 AGO2, 11A9 Sigma SAB4200085 IP (10ug) ,IB (1:1000) human 8 AGO2 EIF2C2 Sino Biologicals 50683-R036 IB (1:2000), IHC (1:100), PLA (1:200) human 9 Anti- EGFR ( phospho Y1092) Abcam ab40815 ( EP774Y) IHC (1:100) human, mouse 10 Phospho-EGF Receptor (Y1068) Cell Signaling 2234S IB (1:1000) human, mouse 11 Phospho-p44/42 MAPK (Erk 1/2) Cell Signaling 4376 IB (1:1000) human, mouse 12 ERK1(K-23) Santa Cruz sc-94 IB (1:500) human, mouse, rat 13 Total p44/42 MAPK (Erk 1/2) Cell Signaling 9102 IB (1:1000) human, mouse 14 EGFR Antibody Abcam ab52894 ( EP38Y) IB (1:1000),IHC (1:50) human, mouse, rat 15 EGFR(1005) Santa Cruz sc-03 IB (1:500) human, mouse, rat 16 Anti-FLAG antibody Sigma F7425 IP (4ug), IB (1:1000) human, mouse 17 Phospho-Akt S473 Cell Signaling 4060S IB (1:1000) human,mouse 18 AKT (pan) - C67E7 Cell Signaling 4691 IB (1:1000) human,mouse 19 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:1000) mouse 20 E-cadherin (36) Ventana Roche 790-4497 IF ( 1:100) mouse 21 Nk1.1 (PK136) Invitrogen MA1-70100 IHC (1:100) mouse 22 anti-CD4 (SP35) Ventana 790-4423 IHC (1:100) mouse 23 anti-CD68 (KP-1) Ventana 790-2931 IHC (1:100) mouse 24 anti-CD8 (SP57) Ventana 790-4460 IHC (1:100) mouse 25 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:500) mouse 26 GAPDH- HRP Cell Signaling 3683 IB (1:5000) human, mouse 27 Normal mouse IgG Santa Cruz sc-2025 Isotype Control IP mouse 28 Normal Rat IgG Abcam ab18450 Isotype Control IP rat 29 Normal Rabbit IgG Millipore 12-370 Isotype Control IP rabbit IP: Immunoprecipitation; IB: Immunoblot; IHC: Immunohistochemistry; PLA: Proximity Ligation Assay Validation of the antibodies used in the study: References and links cited below show specificity and use of the respective antibody for various applications. ..
Immunohistochemistry:Article Title: An essential role for Argonaute 2 in EGFR-KRAS signaling in pancreatic cancer development
Article Snippet: .. Supplementary Table 1: Antibodies used in the study # Antibody Vendor Catalog Number Application Specificity 1 Anti-Ras clone 10 (RAS10) Millipore 05-516 IP (4ug), IB (1:1000), PLA (1:250) human, mouse 2 K-Ras-2B Antibody (C-19) Santa Cruz sc-521 IP (4ug) human 3 K-Ras monoclonal Ab Santa Cruz sc-30 IB (1:200) human, mouse 4 RAS (G12D Mutant Specific) DH87 Cell Signaling 14429S IB (1:1000) human 5 KRAS (3B10-2F2) Sigma- aldrich WH0003845M1 IB (1:1000) Human 6 HRAS- specific Antibody Proteintech 18295-1-AP IB (1:1000) human, mouse, rat 7 AGO2, 11A9 Sigma SAB4200085 IP (10ug) ,IB (1:1000) human 8 AGO2 EIF2C2 Sino Biologicals 50683-R036 IB (1:2000), IHC (1:100), PLA (1:200) human 9 Anti- EGFR ( phospho Y1092) Abcam ab40815 ( EP774Y) IHC (1:100) human, mouse 10 Phospho-EGF Receptor (Y1068) Cell Signaling 2234S IB (1:1000) human, mouse 11 Phospho-p44/42 MAPK (Erk 1/2) Cell Signaling 4376 IB (1:1000) human, mouse 12 ERK1(K-23) Santa Cruz sc-94 IB (1:500) human, mouse, rat 13 Total p44/42 MAPK (Erk 1/2) Cell Signaling 9102 IB (1:1000) human, mouse 14 EGFR Antibody Abcam ab52894 ( EP38Y) IB (1:1000),IHC (1:50) human, mouse, rat 15 EGFR(1005) Santa Cruz sc-03 IB (1:500) human, mouse, rat 16 Anti-FLAG antibody Sigma F7425 IP (4ug), IB (1:1000) human, mouse 17 Phospho-Akt S473 Cell Signaling 4060S IB (1:1000) human,mouse 18 AKT (pan) - C67E7 Cell Signaling 4691 IB (1:1000) human,mouse 19 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:1000) mouse 20 E-cadherin (36) Ventana Roche 790-4497 IF ( 1:100) mouse 21 Nk1.1 (PK136) Invitrogen MA1-70100 IHC (1:100) mouse 22 anti-CD4 (SP35) Ventana 790-4423 IHC (1:100) mouse 23 anti-CD68 (KP-1) Ventana 790-2931 IHC (1:100) mouse 24 anti-CD8 (SP57) Ventana 790-4460 IHC (1:100) mouse 25 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:500) mouse 26 GAPDH- HRP Cell Signaling 3683 IB (1:5000) human, mouse 27 Normal mouse IgG Santa Cruz sc-2025 Isotype Control IP mouse 28 Normal Rat IgG Abcam ab18450 Isotype Control IP rat 29 Normal Rabbit IgG Millipore 12-370 Isotype Control IP rabbit IP: Immunoprecipitation; IB: Immunoblot; IHC: Immunohistochemistry; PLA: Proximity Ligation Assay Validation of the antibodies used in the study: References and links cited below show specificity and use of the respective antibody for various applications. ..
Control:Article Title: An essential role for Argonaute 2 in EGFR-KRAS signaling in pancreatic cancer development
Article Snippet: .. Supplementary Table 1: Antibodies used in the study # Antibody Vendor Catalog Number Application Specificity 1 Anti-Ras clone 10 (RAS10) Millipore 05-516 IP (4ug), IB (1:1000), PLA (1:250) human, mouse 2 K-Ras-2B Antibody (C-19) Santa Cruz sc-521 IP (4ug) human 3 K-Ras monoclonal Ab Santa Cruz sc-30 IB (1:200) human, mouse 4 RAS (G12D Mutant Specific) DH87 Cell Signaling 14429S IB (1:1000) human 5 KRAS (3B10-2F2) Sigma- aldrich WH0003845M1 IB (1:1000) Human 6 HRAS- specific Antibody Proteintech 18295-1-AP IB (1:1000) human, mouse, rat 7 AGO2, 11A9 Sigma SAB4200085 IP (10ug) ,IB (1:1000) human 8 AGO2 EIF2C2 Sino Biologicals 50683-R036 IB (1:2000), IHC (1:100), PLA (1:200) human 9 Anti- EGFR ( phospho Y1092) Abcam ab40815 ( EP774Y) IHC (1:100) human, mouse 10 Phospho-EGF Receptor (Y1068) Cell Signaling 2234S IB (1:1000) human, mouse 11 Phospho-p44/42 MAPK (Erk 1/2) Cell Signaling 4376 IB (1:1000) human, mouse 12 ERK1(K-23) Santa Cruz sc-94 IB (1:500) human, mouse, rat 13 Total p44/42 MAPK (Erk 1/2) Cell Signaling 9102 IB (1:1000) human, mouse 14 EGFR Antibody Abcam ab52894 ( EP38Y) IB (1:1000),IHC (1:50) human, mouse, rat 15 EGFR(1005) Santa Cruz sc-03 IB (1:500) human, mouse, rat 16 Anti-FLAG antibody Sigma F7425 IP (4ug), IB (1:1000) human, mouse 17 Phospho-Akt S473 Cell Signaling 4060S IB (1:1000) human,mouse 18 AKT (pan) - C67E7 Cell Signaling 4691 IB (1:1000) human,mouse 19 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:1000) mouse 20 E-cadherin (36) Ventana Roche 790-4497 IF ( 1:100) mouse 21 Nk1.1 (PK136) Invitrogen MA1-70100 IHC (1:100) mouse 22 anti-CD4 (SP35) Ventana 790-4423 IHC (1:100) mouse 23 anti-CD68 (KP-1) Ventana 790-2931 IHC (1:100) mouse 24 anti-CD8 (SP57) Ventana 790-4460 IHC (1:100) mouse 25 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:500) mouse 26 GAPDH- HRP Cell Signaling 3683 IB (1:5000) human, mouse 27 Normal mouse IgG Santa Cruz sc-2025 Isotype Control IP mouse 28 Normal Rat IgG Abcam ab18450 Isotype Control IP rat 29 Normal Rabbit IgG Millipore 12-370 Isotype Control IP rabbit IP: Immunoprecipitation; IB: Immunoblot; IHC: Immunohistochemistry; PLA: Proximity Ligation Assay Validation of the antibodies used in the study: References and links cited below show specificity and use of the respective antibody for various applications. ..
Immunoprecipitation:Article Title: An essential role for Argonaute 2 in EGFR-KRAS signaling in pancreatic cancer development
Article Snippet: .. Supplementary Table 1: Antibodies used in the study # Antibody Vendor Catalog Number Application Specificity 1 Anti-Ras clone 10 (RAS10) Millipore 05-516 IP (4ug), IB (1:1000), PLA (1:250) human, mouse 2 K-Ras-2B Antibody (C-19) Santa Cruz sc-521 IP (4ug) human 3 K-Ras monoclonal Ab Santa Cruz sc-30 IB (1:200) human, mouse 4 RAS (G12D Mutant Specific) DH87 Cell Signaling 14429S IB (1:1000) human 5 KRAS (3B10-2F2) Sigma- aldrich WH0003845M1 IB (1:1000) Human 6 HRAS- specific Antibody Proteintech 18295-1-AP IB (1:1000) human, mouse, rat 7 AGO2, 11A9 Sigma SAB4200085 IP (10ug) ,IB (1:1000) human 8 AGO2 EIF2C2 Sino Biologicals 50683-R036 IB (1:2000), IHC (1:100), PLA (1:200) human 9 Anti- EGFR ( phospho Y1092) Abcam ab40815 ( EP774Y) IHC (1:100) human, mouse 10 Phospho-EGF Receptor (Y1068) Cell Signaling 2234S IB (1:1000) human, mouse 11 Phospho-p44/42 MAPK (Erk 1/2) Cell Signaling 4376 IB (1:1000) human, mouse 12 ERK1(K-23) Santa Cruz sc-94 IB (1:500) human, mouse, rat 13 Total p44/42 MAPK (Erk 1/2) Cell Signaling 9102 IB (1:1000) human, mouse 14 EGFR Antibody Abcam ab52894 ( EP38Y) IB (1:1000),IHC (1:50) human, mouse, rat 15 EGFR(1005) Santa Cruz sc-03 IB (1:500) human, mouse, rat 16 Anti-FLAG antibody Sigma F7425 IP (4ug), IB (1:1000) human, mouse 17 Phospho-Akt S473 Cell Signaling 4060S IB (1:1000) human,mouse 18 AKT (pan) - C67E7 Cell Signaling 4691 IB (1:1000) human,mouse 19 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:1000) mouse 20 E-cadherin (36) Ventana Roche 790-4497 IF ( 1:100) mouse 21 Nk1.1 (PK136) Invitrogen MA1-70100 IHC (1:100) mouse 22 anti-CD4 (SP35) Ventana 790-4423 IHC (1:100) mouse 23 anti-CD68 (KP-1) Ventana 790-2931 IHC (1:100) mouse 24 anti-CD8 (SP57) Ventana 790-4460 IHC (1:100) mouse 25 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:500) mouse 26 GAPDH- HRP Cell Signaling 3683 IB (1:5000) human, mouse 27 Normal mouse IgG Santa Cruz sc-2025 Isotype Control IP mouse 28 Normal Rat IgG Abcam ab18450 Isotype Control IP rat 29 Normal Rabbit IgG Millipore 12-370 Isotype Control IP rabbit IP: Immunoprecipitation; IB: Immunoblot; IHC: Immunohistochemistry; PLA: Proximity Ligation Assay Validation of the antibodies used in the study: References and links cited below show specificity and use of the respective antibody for various applications. ..
Western Blot:Article Title: An essential role for Argonaute 2 in EGFR-KRAS signaling in pancreatic cancer development
Article Snippet: .. Supplementary Table 1: Antibodies used in the study # Antibody Vendor Catalog Number Application Specificity 1 Anti-Ras clone 10 (RAS10) Millipore 05-516 IP (4ug), IB (1:1000), PLA (1:250) human, mouse 2 K-Ras-2B Antibody (C-19) Santa Cruz sc-521 IP (4ug) human 3 K-Ras monoclonal Ab Santa Cruz sc-30 IB (1:200) human, mouse 4 RAS (G12D Mutant Specific) DH87 Cell Signaling 14429S IB (1:1000) human 5 KRAS (3B10-2F2) Sigma- aldrich WH0003845M1 IB (1:1000) Human 6 HRAS- specific Antibody Proteintech 18295-1-AP IB (1:1000) human, mouse, rat 7 AGO2, 11A9 Sigma SAB4200085 IP (10ug) ,IB (1:1000) human 8 AGO2 EIF2C2 Sino Biologicals 50683-R036 IB (1:2000), IHC (1:100), PLA (1:200) human 9 Anti- EGFR ( phospho Y1092) Abcam ab40815 ( EP774Y) IHC (1:100) human, mouse 10 Phospho-EGF Receptor (Y1068) Cell Signaling 2234S IB (1:1000) human, mouse 11 Phospho-p44/42 MAPK (Erk 1/2) Cell Signaling 4376 IB (1:1000) human, mouse 12 ERK1(K-23) Santa Cruz sc-94 IB (1:500) human, mouse, rat 13 Total p44/42 MAPK (Erk 1/2) Cell Signaling 9102 IB (1:1000) human, mouse 14 EGFR Antibody Abcam ab52894 ( EP38Y) IB (1:1000),IHC (1:50) human, mouse, rat 15 EGFR(1005) Santa Cruz sc-03 IB (1:500) human, mouse, rat 16 Anti-FLAG antibody Sigma F7425 IP (4ug), IB (1:1000) human, mouse 17 Phospho-Akt S473 Cell Signaling 4060S IB (1:1000) human,mouse 18 AKT (pan) - C67E7 Cell Signaling 4691 IB (1:1000) human,mouse 19 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:1000) mouse 20 E-cadherin (36) Ventana Roche 790-4497 IF ( 1:100) mouse 21 Nk1.1 (PK136) Invitrogen MA1-70100 IHC (1:100) mouse 22 anti-CD4 (SP35) Ventana 790-4423 IHC (1:100) mouse 23 anti-CD68 (KP-1) Ventana 790-2931 IHC (1:100) mouse 24 anti-CD8 (SP57) Ventana 790-4460 IHC (1:100) mouse 25 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:500) mouse 26 GAPDH- HRP Cell Signaling 3683 IB (1:5000) human, mouse 27 Normal mouse IgG Santa Cruz sc-2025 Isotype Control IP mouse 28 Normal Rat IgG Abcam ab18450 Isotype Control IP rat 29 Normal Rabbit IgG Millipore 12-370 Isotype Control IP rabbit IP: Immunoprecipitation; IB: Immunoblot; IHC: Immunohistochemistry; PLA: Proximity Ligation Assay Validation of the antibodies used in the study: References and links cited below show specificity and use of the respective antibody for various applications. ..
Biomarker Discovery:Article Title: An essential role for Argonaute 2 in EGFR-KRAS signaling in pancreatic cancer development
Article Snippet: .. Supplementary Table 1: Antibodies used in the study # Antibody Vendor Catalog Number Application Specificity 1 Anti-Ras clone 10 (RAS10) Millipore 05-516 IP (4ug), IB (1:1000), PLA (1:250) human, mouse 2 K-Ras-2B Antibody (C-19) Santa Cruz sc-521 IP (4ug) human 3 K-Ras monoclonal Ab Santa Cruz sc-30 IB (1:200) human, mouse 4 RAS (G12D Mutant Specific) DH87 Cell Signaling 14429S IB (1:1000) human 5 KRAS (3B10-2F2) Sigma- aldrich WH0003845M1 IB (1:1000) Human 6 HRAS- specific Antibody Proteintech 18295-1-AP IB (1:1000) human, mouse, rat 7 AGO2, 11A9 Sigma SAB4200085 IP (10ug) ,IB (1:1000) human 8 AGO2 EIF2C2 Sino Biologicals 50683-R036 IB (1:2000), IHC (1:100), PLA (1:200) human 9 Anti- EGFR ( phospho Y1092) Abcam ab40815 ( EP774Y) IHC (1:100) human, mouse 10 Phospho-EGF Receptor (Y1068) Cell Signaling 2234S IB (1:1000) human, mouse 11 Phospho-p44/42 MAPK (Erk 1/2) Cell Signaling 4376 IB (1:1000) human, mouse 12 ERK1(K-23) Santa Cruz sc-94 IB (1:500) human, mouse, rat 13 Total p44/42 MAPK (Erk 1/2) Cell Signaling 9102 IB (1:1000) human, mouse 14 EGFR Antibody Abcam ab52894 ( EP38Y) IB (1:1000),IHC (1:50) human, mouse, rat 15 EGFR(1005) Santa Cruz sc-03 IB (1:500) human, mouse, rat 16 Anti-FLAG antibody Sigma F7425 IP (4ug), IB (1:1000) human, mouse 17 Phospho-Akt S473 Cell Signaling 4060S IB (1:1000) human,mouse 18 AKT (pan) - C67E7 Cell Signaling 4691 IB (1:1000) human,mouse 19 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:1000) mouse 20 E-cadherin (36) Ventana Roche 790-4497 IF ( 1:100) mouse 21 Nk1.1 (PK136) Invitrogen MA1-70100 IHC (1:100) mouse 22 anti-CD4 (SP35) Ventana 790-4423 IHC (1:100) mouse 23 anti-CD68 (KP-1) Ventana 790-2931 IHC (1:100) mouse 24 anti-CD8 (SP57) Ventana 790-4460 IHC (1:100) mouse 25 Anti-Cytokeratin 19 antibody Abcam ab133496 IHC (1:500) mouse 26 GAPDH- HRP Cell Signaling 3683 IB (1:5000) human, mouse 27 Normal mouse IgG Santa Cruz sc-2025 Isotype Control IP mouse 28 Normal Rat IgG Abcam ab18450 Isotype Control IP rat 29 Normal Rabbit IgG Millipore 12-370 Isotype Control IP rabbit IP: Immunoprecipitation; IB: Immunoblot; IHC: Immunohistochemistry; PLA: Proximity Ligation Assay Validation of the antibodies used in the study: References and links cited below show specificity and use of the respective antibody for various applications. ..
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